Repeat the same process with the remaining test tube, taking 1 ml from the previous tube and adding to the next 9 ml diluents.The second tube now has a total dilution factor of 10 -2.Then dilute it and pour into a second test tube.Now, take 1 ml of mixture from the 10 -1 concentration test tube.Discharge a tip of pipette, and insert a new pipe tip into the pipette.Dilution is thoroughly mixed by removing and filling the pipette several times.This provides an initial dilution of 10 -1. Then add sample solution to the first tube to form a total volume of 10 ml.Add 1 ml of a well-mixed sample/culture is drawn into the pipette.Take a given sample/culture in a test tube and six test tubes, each containing 9 ml of sterile diluent, which can be pure water or 0.9% saline.The following is a ten-step purification process for a 10 -6 purification factor.
#Serial dilution serial#
The serial dilution formula for the dilution factor of each tube is –.The dilution factor in serial dilution can be determined either by an individual test tube or can be counted as a complete filtering element throughout the series.Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes in the laboratory. A serial dilution is a series of dilutions made sequentially, using the same dilution factor for each step.
On the other hand, for a less contaminated sample, a low dilution factor might be sufficient. For example, if a water sample is taken from a very polluted environment, the dilution factor is increased.
0 kommentar(er)
